Services
• Apoptosis assay using Annexin V and Propidum iodide, detection by fluorescent imaging or by FlowCytomtery.
• Cell cycle analysis by flow cytometry to identify cells in different stages of division. Cell cycle phases.
• In vitro cell migration and Chemotaxis assays. 
• In vitro Drug release testing for polymeric particulate systems, the drug withdraw is assessed by Fluorescent imaging for viable and fixed cells using a confocal microscope.
• In vitro mitochondrial tracking assays.
• Wound Healing Assay to study the migratory response of endothelial cells to angiogenic inducers or inhibitors.
• Trans well assay to study the migratory response of endothelial cells to angiogenic inducers or inhibitors.
• In vitro endothelial cell tube formation assay, to study the angiogenesis function.
• Antibacterial assays.

Application Areas

• Isolation of cells
Several methods exist for isolating cells from tissues for ex vivo culture. Blood cells can be easily extracted and purified; however, only the white cells can develop in culture. Enzymatic digestion by enzymes such as collagenase, trypsin, or protease, which break down the extracellular matrix, can liberate mononuclear cells from soft tissues. Alternatively, tissue sections such as cord tissue, placenta, endothelium, and bone marrow can be placed in growth media and the cells that grow out can be cultured.
Mesenchymal stem cells are one of our Mammalian cell isolation experiences (MSCs). Adult tissues such as bone marrow, adipose tissue, inner organs, and blood vessels, as well as "young sources" such as amniotic fluid, amniotic membrane, umbilical cord, or placenta, are utilized to isolate MSCs. Furthermore, dental MSCs are isolated from the apical papilla, periodontal ligament, tooth pulp, and gingival tissue.
• Culture of Primary Cells
Primary cells are extracted and sub-cultured directly from living tissue to replicate and maintain the physiology of biological functions in vitro. They are becoming more widely used in life science research for a variety of purposes, including pathophysiology testing, medication toxicity investigations, and pharmaceutical development.
Global Labs has optimized the isolation, purification, subculture, and growth of primary cell types to produce grown cells that promise purity, low passage, thorough characterization, and quality check control.
• Cell-based assays
Cellular assays, also known as cell-based assays, are useful for determining cell viability, proliferation, migration, invasion, chemotaxis, apoptosis, angiogenesis, oxidative stress, cell signaling, and live-cell analysis.
• Live Cell Imaging and Image Analysis
At Global Research labs, we investigate the dynamic cellular activities in real-time using live-cell imaging and analysis to get unique insights into biology. These methods combine with PCR, flow cytometry, immunocytochemistry, and tissue labeling with antibodies, which take an image of cellular activities. Cameras, live-cell fluorescent dyes, fluorescent proteins, microscopy have all improved our ability to observe and understand living cells, their intercellular interactions, and intracellular activities with astonishing detail and fidelity.
• Health Analysis and Cell Counting
The Trypan blue dye using haemocytometer and 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) calorimetric cell viability and proliferation tests are two the two applied techniques at Global Research Labs for counting cells and evaluating cell health and viability in culture.
• Cell Apoptosis assessment
One of the most important steps in confirming a drug's or compound's cytotoxic action is to detect cell apoptosis. We use Flowcytometry to identify apoptotic cells utilizing Annexin V fluorescent conjugates and propidium iodide staining. The technique is based on the fact that annexin V, a 35–36 kDa Ca2+-dependent phospholipid-binding protein with a high affinity for the anionic phospholipid phosphatidylserine, is a 35–36 kDa Ca2+-dependent phospholipid-binding protein with a high affinity for the anionic phospholipid phosphatidylserine (PS). PS is found on the cytoplasmic surface of the plasma membrane in healthy cells. The plasma membrane undergoes structural changes during apoptosis, including the translocation of PS from the inner to the outer leaflet (extracellular side) of the plasma membrane, where it binds the conjugated antibody and is measured by Flowcytometry. The externalisation of phosphatidylserine, a marker of intermediate stages of apoptosis, can be studied using these annexin V conjugates, which allow quick and reliable detection methods. Flow cytometry typically shows a 100-fold difference in fluorescence intensity between apoptotic and nonapoptotic cells treated with our fluorescent annexin V conjugates.
• Assay for the Cell Cycle
One of the earliest applications of flow cytometry was cell cycle analysis by DNA content quantification. A number of DNA binding dyes can be used to stain the DNA of human, yeast, plant, or bacterial cells. For cell cycle study, flow cytometry can be used to estimate the percentages of a cell population in each phase of the cell cycle was measured at Global Research Labs using Propidium iodide (PI) dye for quantifying DNA content. PI, on the other hand, cannot pass an intact plasma membrane, hence it will only be found in the DNA of cells whose plasma membrane has been compromised/permeabilized. Microscopy, where PI may be seen using a rhodamine(red) filter (= 536/617), or Flow Cytometry analysis, where PI staining can be tracked in the FL2 channel, are both options for detection and analysis.
• Toxicity Testing
The impact of novel medications, cosmetics, and chemicals on the survival and growth of a variety of cell types is studied using cell culture. The maximum permitted dosage of new medications is also determined using cultured cells such as cancer cells and immortalised cell lines. The medication's half-maximum inhibitory effect (IC50), cytotoxic MTT assay, tracking of fluorescently labeled drug release, and quantification of the percentage of apoptotic cells were all used at Global Research Labs to make the assessment.
• Gene expression alterations in vitro and genetic engineering
In order to investigate the effect of stimulating or knocking down distinct genes on cultured cell proliferation, the genetic profile of genes could be manipulated in cultured cells. We provide cell engineering technology using a variety of methodologies, including transcriptional suppression with short interference RNA (si-RNA), miRNA inhibitor, transcription activation with plasmid vector transfection, cloning, and miRNA mimic.
Furthermore, gene-editing technology emerge a new era in cancer and genetic disease management. We used clustered regularly interspaced short palindromic repeats (CRISPR) in a genetic engineering project at Global laboratories. CRISPR-Cas9 is a breakthrough technique that allows geneticists and medical researchers to edit regions of the genome by removing, inserting, or modifying DNA sequences. It is the simplest, most adaptable, most exact approach of genetic manipulation now available, and it is making a storm in the scientific community. Finally, the efficiency of the genetically altered cells is confirmed by PCR gene identification and Western blotting protein detection.
• In vitro Gene Therapy Experiments
Global Research Labs provide a good experience in experimental gene and cell-based therapy. We applied a DNA vector, in which a functioning gene is inserted into the genome of the DNA vector. These genetically engineered cells are then transfected into the cultured cells (in-vitro) or experimental animal model (in-vivo).
One of the most well-known uses of modified cell therapy research undertaken at Global laboratories is Chimeric Antigen Receptor T-cells (CAR T cells). T-cell therapy (CAR) is a method of modifying immune cells called T cells (a kind of white blood cell) in the lab such that they can find and destroy cancer cells. In vitro and in vivo tests are used to test the genetically modified T cells.

Lab Equipment
• Co2 Incubator
• Safety cabinet
• Microscope with Olympus Camera
• Centrifuges