Description:
The workshop is designed to provide a solid understanding of cell culture through classroom presentation and laboratory work. Clients will gain significant experience in the manipulation of stem cells through culture. Through integrated learning methods, utilizing hands-on training to reinforce lecture material participants will learn the biological basis and relevance of the process of culturing cells.
Topics that will be covered:
The intensive workshop is specifically designed to address
• Introduction to stem cells isolation from different sources
• Primary cell culture. Conditioning media, Antibiotics.
• Harvesting of cultured cells, cell counting and viability determination
• development of growth curves, growth of adherent and suspension cells
• cryopreservation and cryothawing of cells
Duration: 4 working days (3 hours/day).
Course structure: The course will constitute theoretical classes, demonstration and hand training.
Workshop Agenda:
1st day: Introduction in hematopoietic stem cells (HSCs) isolation and purification
2nd day: basic cell culture techniques, preparation cell culture media.
3rd day: Maintenance and sub culturing of the human/cancer cell lines for various experiments, transfection technologies and gene/protein expression
4th day: cell counting and viability determination, Cell death assay and cell preservation and retrieval.
Who Should Attend:
The workshops were designed for both beginners and those who are already working with the techniques but wishing to learn appropriate technical skills that can be applied in their practical field. Moreover; to learn and trained on new era of research technology.
Who will attend?
• Master/Ph.D. Students, Scientists
• Researchers
• Research Assistants, Technical Officers
• Academic Staff
• Undergraduate students of medical and para-medical specialities.
Supplements:
At the end of the workshop; attendees will receive the following documents:
• Official training Certificate (Signed & stamped).
• Full workshop review (PDF).
• Technical data troubleshooting memo (PDF).
• In addition, the participants will have a chance to win a prize on participation of a ‘quiz’ based on the knowledge gained during the workshop