Description:
Chimeric antigen receptors (CAR) are engineered T cell receptors that redirect T cells to recognize and destroy cancer cell targets. CAR T cells are referred to as chimeric because they are engineered from parts that originate within different sources. This practiacl course will provide you with a basic understanding of principles for the construction of CAR-T cell for research purpose.
Topics that will be covered:
• T cell isolation using magnetic beads & characterization.
• Genetic Engineering of CAR-T.
• Deliver of CAR-T plasmid into T-Cell with CRISPR-Cas9.
• Co-culture & analysis of cell killing effect.
Duration: 5 working days (4 hours/day).
Course structure: The course will constitute theoretical classes, demonstration and hand training.
Workshop Agenda:
1st day: Introduction on T- cell isolation from PMNLs, activation, expansion and cultivation
2nd day: Isolation of CD3 positive cells and PBMCs using Ficoll-Paque.
3rd day: T cell expansion, cultivation and activation using Dynabeads
4th day: introduction on the Bioinformatics background for design an experiment for CAR T- cell (Demo only)
5th day: Assessing CAR T cell functionality and interaction with tumor cells by qPCR and Immunofluorescence
Who Should Attend:
The workshops were designed for both beginners and those who are already working with the techniques but wishing to learn appropriate technical skills that can be applied in their practical field. Moreover; to learn and trained on new era of research technology.
Who will attend?
• Master/Ph.D. Students, Scientists
• Researchers
• Research Assistants, Technical Officers
• Academic Staff
• Undergraduate students of medical and para-medical specialities.
Supplements:
At the end of the workshop; attendees will receive the following documents:
• Official training Certificate (Signed & stamped).
• Full workshop review (PDF).
• Technical data troubleshooting memo (PDF).
• In addition, the participants will have a chance to win a prize on participation of a ‘quiz’ based on the knowledge gained during the workshop